Rootine is built on 10+ years of nutrigenetic science, technology, and algorithm / AI development.
Science
Extraordinary Science for Extraordinary People

The Data We Use

Each type of data plays a role in determining your unique nutrient needs. And, data points are used to contextualize each other and build a model to accurately determine the type and amount of each nutrient required to meet your ideal daily intake.

Lifestyle Test

Our 19-question analysis provides information about your size, age, biological gender, activity level, dietary preferences, family life, and more. The data is used directionally to create your baseline range for each nutrient.

For example, if you eat a vegan diet, we know you likely need more vitamin B12. [1, 2, 3]. If you avoid dairy, we know you likely need more calcium. [4, 5, 6]. And If you are a female and have a heavy menstrual flow, we know you likely need more iron. [7, 8, 9]

DNA

The science of nutrigenetics details how slight variations in your genes can alter how you absorb, metabolize, distribute, and excrete nutrients, thus impacting the type and amount of each nutrient you need. We consider 50+ research-proven genetic variations.

For example, a variation in the NQO1 gene makes your body less efficient at activating CoQ10. We correct by increasing other antioxidants in your formula. [10, 11, 12, 13, 14]. And, a variation in the VDR gene makes vitamin D bind less efficiently to its receptor. We correct by increasing your vitamin D to achieve a normal level of receptor activation. [15, 16, 17, 18, 19]

Blood

Blood nutrient levels have been the gold standard data source in the medical community in terms of recommending supplementation. Changes (or lack of positive change) over recurrent blood tests is also used to determine if the dose needs further increase, a decrease, or if it is at the ideal amount.

  1. Pawlak R, Parrott SJ, Raj S, Cullum-Dugan D, Lucus D. How prevalent is vitamin B(12) deficiency among vegetarians? Nutr Rev. 2013 Feb;71(2):110-7. doi: 10.1111/nure.12001. Epub 2013 Jan 2. PMID: 23356638.
  2. Woo KS, Kwok TC, Celermajer DS. Vegan diet, subnormal vitamin B-12 status and cardiovascular health. Nutrients. 2014;6(8):3259-3273. Published 2014 Aug 19. doi:10.3390/nu6083259
  3. Lederer AK, Hannibal L, Hettich M, Behringer S, Spiekerkoetter U, Steinborn C, Gründemann C, Zimmermann-Klemd AM, Müller A, Simmet T, Schmiech M, Maul-Pavicic A, Samstag Y, Huber R. Vitamin B12 Status Upon Short-Term Intervention with a Vegan Diet-A Randomized Controlled Trial in Healthy Participants. Nutrients. 2019 Nov 18;11(11):2815. doi: 10.3390/nu11112815. PMID: 31752105; PMCID: PMC6893687.
  4. Gao X, Wilde PE, Lichtenstein AH, Tucker KL. Meeting adequate intake for dietary calcium without dairy foods in adolescents aged 9 to 18 years (National Health and Nutrition Examination Survey 2001-2002). J Am Diet Assoc. 2006 Nov;106(11):1759-65. doi: 10.1016/j.jada.2006.08.019. PMID: 17081826.
  5. Hodges JK, Cao S, Cladis DP, Weaver CM. Lactose Intolerance and Bone Health: The Challenge of Ensuring Adequate Calcium Intake. Nutrients. 2019;11(4):718. Published 2019 Mar 28. doi:10.3390/nu11040718
  6. Rozenberg S, Body JJ, Bruyère O, et al. Effects of Dairy Products Consumption on Health: Benefits and Beliefs--A Commentary from the Belgian Bone Club and the European Society for Clinical and Economic Aspects of Osteoporosis, Osteoarthritis and Musculoskeletal Diseases. Calcif Tissue Int. 2016;98(1):1-17. doi:10.1007/s00223-015-0062-x
  7. Arvidsson B, Ekenved G, Rybo G, Sölvell L. Iron prophylaxis in menorrhagia. Acta Obstet Gynecol Scand. 1981;60(2):157-60. PMID: 7246080.
  8. Mishra V, Verneker R, Gandhi K, Choudhary S, Lamba S. Iron Deficiency Anemia with Menorrhagia: Ferric Carboxymaltose a Safer Alternative to Blood Transfusion. J Midlife Health. 2018;9(2):92-96. doi:10.4103/jmh.JMH_121_17
  9. Mansour D, Hofmann A, Gemzell-Danielsson K. A Review of Clinical Guidelines on the Management of Iron Deficiency and Iron-Deficiency Anemia in Women with Heavy Menstrual Bleeding. Adv Ther. 2021 Jan;38(1):201-225. doi: 10.1007/s12325-020-01564-y. Epub 2020 Nov 27. PMID: 33247314; PMCID: PMC7695235.
  10. Fischer A, Schmelzer C, Rimbach G, Niklowitz P, Menke T, Döring F. Association between genetic variants in the Coenzyme Q10 metabolism and Coenzyme Q10 status in humans. BMC Res Notes. 2011;4:245. Published 2011 Jul 21. doi:10.1186/1756-0500-4-245
  11. Freriksen JJ, Salomon J, Roelofs HM, et al. Genetic polymorphism 609C>T in NAD(P)H:quinone oxidoreductase 1 enhances the risk of proximal colon cancer. J Hum Genet. 2014;59(7):381‐386. doi:10.1038/jhg.2014.38
  12. Yu H, Liu H, Wang LE, Wei Q. A functional NQO1 609C>T polymorphism and risk of gastrointestinal cancers: a meta-analysis. PLoS One. 2012;7(1):e30566. doi:10.1371/journal.pone.0030566
  13. Yadav U, Kumar P, Rai V. "NQO1 Gene C609T Polymorphism (dbSNP: rs1800566) and Digestive Tract Cancer Risk: A Meta-Analysis.". Nutr Cancer. 2018;70(4):557‐568. doi:10.1080/01635581.2018.1460674
  14. Ding R, Lin S, Chen D. Association of NQO1 rs1800566 polymorphism and the risk of colorectal cancer: a meta-analysis. Int J Colorectal Dis. 2012;27(7):885‐892. doi:10.1007/s00384-011-1396-0
  15. Palomba S, Orio F Jr, Russo T, et al. BsmI vitamin D receptor genotypes influence the efficacy of antiresorptive treatments in postmenopausal osteoporotic women. A 1-year multicenter, randomized and controlled trial. Osteoporos Int. 2005;16(8):943‐952. doi:10.1007/s00198-004-1800-5
  16. Jia F, Sun RF, Li QH, et al. Vitamin D receptor BsmI polymorphism and osteoporosis risk: a meta-analysis from 26 studies. Genet Test Mol Biomarkers. 2013;17(1):30‐34. doi:10.1089/gtmb.2012.0267
  17. Palomba S, Numis FG, Mossetti G, et al. Raloxifene administration in post-menopausal women with osteoporosis: effect of different BsmI vitamin D receptor genotypes. Hum Reprod. 2003;18(1):192‐198. doi:10.1093/humrep/deg031
  18. Creatsa M, Pliatsika P, Kaparos G, et al. The effect of vitamin D receptor BsmI genotype on the response to osteoporosis treatment in postmenopausal women: a pilot study. J Obstet Gynaecol Res. 2011;37(10):1415‐1422. doi:10.1111/j.1447-0756.2011.01557.
  19. Mossetti G, Gennari L, Rendina D, et al. Vitamin D receptor gene polymorphisms predict acquired resistance to clodronate treatment in patients with Paget's disease of bone. Calcif Tissue Int. 2008;83(6):414‐424. doi:10.1007/s00223-008-9193-7.

Expertise


Our Method

A U-shaped curve describes when too much or too little of something has a negative impact. In essence, it describes a nutrient "sweet spot" - which is different for every person. We identify your ideal dose for each nutrient and deliver it in a bespoke daily formula.

The science of nutrigenetics powers our method. Nutrigenetics is the science of how slight genetic variations, inherited from your parents, impact how your body absorbs and metabolizes nutrients.






Tests and Data

Our DNA Test analyzes 50+ SNPs (single nucleotide polymorpisms) that are proven to impact nutrient needs due to the influence on nutrient absorption, distribution, metabolism, excretion, and receptor function. All gene variants adhere to our medical-grade bar of proof to impact nutrient needs: 3 different studies by 3 different researchers in 3 different test populations (3x3x3 min.).

Our Blood Test analyzes blood serum levels of vitamins B6, B9, B12, and D3 to guide initial doses of these nutrients and to determine dose efficacy and adjust formulation with repeated testing. We are expanding our blood testing line shortly.

DNA Tests are analyzed in our licensed medical-genetics laboratory certified ISO 9001, 15189, and 22000 - boasting 100% genetic testing accuracy over 10 years, determined by an external proficiency program. Blood Tests are analyzed in our CLIA-certified, COLA-regulated, high-complexity laboratory. Your data is 100% private and secure.





How We Interpret The Data

We combine peer-reviewed research with clinical expertise to create the data modeling (i.e. algorithm) that generates the bespoke formulas.

In some cases, the analytics process is straightforward; If a user’s blood value of vitamin D is too low ⮕ increase the amount in their formula.

In other scenarios, it is far more complex; the user is a male, vegan, and indicates low energy ⮕ likely indicates an increased need for iron; however, the user has a double HFE gene mutation which makes supplemental iron poisonous ⮕ remove all iron from the formula.

EXAMPLE FORMULATIONMichael C.

Michael’s blood value of Vitamin D is too low, so his formula has an increased amount

Vitamin D3
Phytosterols
Vitamin B2
Omega 3s
Folate (B9)
Coenzyme Q10 (CoQ10)

Overall, our science team utilizes scientific research (and our PhDs) + clinical experience (from our MDs, DOs, RDs, and PharmDs) + sound logic to combine the numerous points of data that influence the final recommended dose for each nutrient.

Nutrients And Delivery

Our nutrient suite consists of 19 time-tested, research-proven nutrients that have been clinically shown to demonstrate safety and efficacy in humans. We choose nutrient forms with the most scientific literature supporting their use while continuing to evaluate newer nutrient forms (e.g. mineral chelates, pre-formed bioactive vitamins, etc.) and introduce them into our suite when sufficient data is available.

More than 3 years of R&D lead to our industry-leading nutrient delivery system: microbeads. This form-factor enables precision dosing to the mcg and the 6+ hour slow-release matrix provides enhanced absorption and maintenance of optimal blood levels.





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